WesternBright Sirius HRP substrate

WesternBright Sirius HRP substrate

  • See bands you couldn't detect before

    WesternBright Sirius is the most sensitive HRP substrate available from Advansta for chemiluminescent Western blotting. With attomole sensitivity and a long-lasting signal, WesternBright Sirius allows you to detect bands not visualized with other substrates. High signal-to-noise and a large dynamic range make it ideal for quantifying low-intensity bands. And, chemifluorescence emission allows WesternBright Sirius to be imaged by chemifluorescence imagers in addition to traditional CCD imagers and film.

  • The most sensitive of Advansta's HRP substrates
    Quantitative signal
    Also emits chemifluorescence for detection using fluorescence imagers

  • Customer feedback

    WesternBright Sirius performed better than Pierce's Femto and had much less background, while maintaining similar (if not better) sensitivity. It is also working better for my specific home-made antibodies. Postdoctoral Researcher, Indiana University

    The WesternBright Sirius substrate has low background and we are able to visualize phosphorylated proteins that we had difficulty seeing with our previous substrates. We have been able to dilute our primary antibodies from 1:1000 to 1:2500 - 1:5000. Graduate Research Associate, Ohio State University

    The signal lasts much longer with WesternBright Sirius, while the Pierce West Femto signal disappeared after a half an hour. Plus, WesternBright Sirius is less expensive! Graduate Research Associate, University of Minnesota